FastQCFastQC Report
Thu 2 Jun 2016
DRR028496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232704
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.18901536784175277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20530.1665444421369607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.076.903341
GTATCAA39750.061.265861
TCAACGC43300.054.6919984
ATCAACG44200.053.742213
CAACGCA44600.053.097845
AACGCAG46450.051.1389856
TATCAAC48800.049.121562
TAGACCG604.9722235E-448.2806055
ACGCAGA52600.044.7467587
CGCAGAG52800.044.165788
GTACATG33550.038.4542121
GCAGAGT62900.035.8074769
TACATGG36100.034.9064752
TCTAGCG850.002756945834.080433
ACCGCTC900.00364800432.187078
ACATGGG37100.032.0135543
GTGGTAT9700.027.6468961
GTACGGA1658.4828775E-526.3348756
CATGGGG23350.024.1919964
TAGTACG3001.727858E-824.1403034
Produced by FastQC (version 0.11.5)