FastQCFastQC Report
Thu 2 Jun 2016
DRR028495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319359
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20550.1557574549459245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.050.1917841
TCAACGC15650.046.6284264
GTATCAA16600.046.604161
ATCAACG16400.044.9332053
CAACGCA17000.042.9272585
AACGCAG18950.038.1120266
GTACATG31400.034.258371
TATCAAC22800.033.609262
CGCAGAG20600.033.11928
ACGCAGA20650.032.4775627
CGATACC7600.031.220022145
TACATGG32300.030.5676962
ACATGGG34300.026.4254423
GCAGAGT25700.023.723689
CATGGGG25050.021.1862774
GTATAAA13150.020.7309631
GTATAGG6150.020.3665431
GAGTACA24000.019.9547181
CGACGAA2257.2638463E-419.17351145
CGTTATA1950.006231890518.8927782
Produced by FastQC (version 0.11.5)