FastQCFastQC Report
Thu 2 Jun 2016
DRR028495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319359
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.1573491369672697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.1388553077668777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.077.7168351
GTATCAA33250.062.1179541
ATCAACG38300.052.9371953
TCAACGC38700.052.577154
CAACGCA40950.049.8651245
AACGCAG42250.048.1594246
TATCAAC43800.046.289832
CGCAGAG48000.041.48528
ACGCAGA48750.040.9954957
CTAGCGT951.036579E-438.11084
GCAGAGT56900.033.5964479
GTACGAC1153.1856302E-431.4828363
GTACATG35350.029.5214041
TCTAGCG1000.00611191428.9642093
TACATGG35450.028.1880192
ACATGGG37850.025.826743
GAGTACA27350.021.463061
CAGAGTA56900.020.9977789
GTGGTAT11400.020.9784321
CATGGGG26350.020.6102054
Produced by FastQC (version 0.11.5)