FastQCFastQC Report
Thu 2 Jun 2016
DRR028494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302786
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22100.1696364560257786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17150.052.0296674
GGTATCA12650.049.5301131
CAACGCA18500.048.2329065
GTATCAA19700.047.8943941
ATCAACG18850.046.931273
GTACATG34150.044.464911
TACATGG34200.042.894562
AACGCAG20600.041.5178346
TATCAAC25200.036.566612
CGCAGAG22500.036.45388
ACATGGG38100.035.7963873
ACGCAGA23050.035.1856587
CATGGGA18350.028.935184
GTACGAC1050.007105637328.0842673
GCAGAGT26300.027.322879
CATGGGG24800.025.8700544
CGATACC9600.023.214834145
GAGTACA23750.022.3465181
TCTATAC8300.022.2051833
TTACGGT1750.003315522321.0698644
Produced by FastQC (version 0.11.5)