FastQCFastQC Report
Thu 2 Jun 2016
DRR028494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302786
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18550.1423871610533119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.13486482046936335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.077.265731
GTATCAA35900.055.119091
TCAACGC40050.048.81784
ATCAACG40200.048.8157773
CAACGCA40800.047.7429285
AACGCAG44550.043.2365346
TATCAAC47650.041.1835062
CGCAGAG53550.035.428998
ACGCAGA54200.034.8705027
GTACATG36450.032.811061
TACATGG38250.030.2904342
GCAGAGT61650.029.012219
ACATGGG39500.027.681963
GTGGTAT10800.026.1742781
CAGAGTA58450.022.919459
TGGTATC11350.022.330022
CATGGGG27450.021.367794
TTACGAT2053.6970995E-421.1940694
AGAGTAC53900.020.9581435
GTACGAG1750.003664401820.709321
Produced by FastQC (version 0.11.5)