FastQCFastQC Report
Thu 2 Jun 2016
DRR028492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384401
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54270.16035333874443364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC30100.053.892374
CAACGCA31400.051.1922875
ATCAACG32250.050.9691623
GTATCAA42600.041.8740271
AACGCAG38350.040.944246
CGCAGAG34950.039.8816688
ACGCAGA40200.037.0450637
GGTATCA26200.036.2933771
GTACATG65500.034.9991951
TATCAAC51650.033.3947262
TACATGG66950.031.2682
ACATGGG66150.030.754713
CATGGGG42850.022.7140314
GCAGAGT54250.022.214719
TCTATAC26050.022.0708583
GAGTACA45350.021.780431
GTATATA38700.021.1422391
GTATAGA28300.020.3164181
GTATTAG29100.019.757891
CATGGGA41150.018.9935914
Produced by FastQC (version 0.11.5)