FastQCFastQC Report
Thu 2 Jun 2016
DRR028492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384401
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.050.3679051
TCAACGC47550.047.968394
ATCAACG48650.047.1814773
CAACGCA49350.045.9253275
GTATCAA56050.042.7982671
AACGCAG57350.039.3927466
CGCAGAG58700.035.8963248
TATCAAC69550.033.940292
ACGCAGA65450.033.5218547
GTACATG64300.032.460511
ACATGGG64950.029.6549743
TACATGG71550.028.4375232
GCAGAGT79400.025.7172019
GGACGGT2559.6159056E-825.5562696
CATGGGA37000.022.505634
CAGAGTA78950.020.177429
GAGTACA47800.020.1649681
GTATATA42350.019.3374331
CATGGGG41400.019.0643184
GTACGGA2450.001227989917.732926
Produced by FastQC (version 0.11.5)