FastQCFastQC Report
Thu 2 Jun 2016
DRR028490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306607
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20930.16018588603918393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13700.054.351624
CAACGCA14050.052.472945
ATCAACG14650.050.81513
GGTATCA10650.048.445951
AACGCAG15550.046.9241836
GTATCAA16250.046.2653471
GTACATG28900.040.806671
ACGCAGA18350.037.3556187
TACATGG30950.036.4367642
CGCAGAG18550.034.446438
TATCAAC21450.034.018642
ACATGGG33400.033.101973
CATGGGA15050.024.4931814
GAGTACA22100.024.0131571
CATGGGG22200.023.5785484
GCAGAGT23900.023.3954859
AGTACCG1600.001973647923.03895
AGTACAT23050.022.064152
TATACTG10000.019.1683645
GTACTAT7100.017.6481671
Produced by FastQC (version 0.11.5)