FastQCFastQC Report
Thu 2 Jun 2016
DRR028490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306607
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.12038815037727488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.11173979628151388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.079.190421
GTATCAA32450.052.9179461
ATCAACG35000.047.99253
TCAACGC35300.047.5846334
CAACGCA36800.045.4482965
AACGCAG38350.043.4226046
TATCAAC41550.040.426892
ACGCAGA44350.037.384827
CGCAGAG45150.036.2413258
GTACATG31500.033.1223561
CCGACCG900.00365263232.178889
TACATGG32850.029.7544422
GCAGAGT53700.029.6620949
ACATGGG32850.027.9912173
CAGAGTA51750.024.7637449
CATGGGG23050.022.9300694
AGGACCG1902.2137555E-422.863945
AATGCGT2603.2430744E-622.2776856
GAGTACA25950.020.6615871
CAGTGCG1800.0043414620.11189
Produced by FastQC (version 0.11.5)