FastQCFastQC Report
Thu 2 Jun 2016
DRR028489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14860
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA620.41722745625841184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.41722745625841184No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA460.3095558546433378No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC310.20861372812920592No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA260.17496635262449528No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA230.1547779273216689No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC210.14131897711978464No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210.14131897711978464No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA210.14131897711978464No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG160.10767160161507401No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC160.10767160161507401No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA150.1009421265141319No Hit
GTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATA150.1009421265141319No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC150.1009421265141319No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCCG100.0069314884145.616449
ATGGGGA405.9948463E-573.564015
TACATGG1050.070.060962
GTACATG1200.061.3033451
CATGGGG659.5433625E-656.5877044
ACATGGG1204.5474735E-1155.173013
GCCCATA400.00559911155.1730081
CCCATAA450.00892081249.0426752
TCAACGC450.00892081249.0426754
CATGGGA709.5167087E-442.0365834
TATGCAG2252.0261739E-47.661261130-134
GATATGC3000.00529584347.1824327145
GACGATA2850.00299009966.048364125-129
Produced by FastQC (version 0.11.5)