FastQCFastQC Report
Thu 2 Jun 2016
DRR028489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14860
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT1751.177658142664872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.6123822341857335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.531628532974428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.4508748317631225No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA610.4104979811574697No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC520.34993270524899056No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA490.32974427994616423No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT480.3230148048452221RNA PCR Primer, Index 31 (95% over 22bp)
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA450.3028263795423957No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA450.3028263795423957No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT380.2557200538358008RNA PCR Primer, Index 31 (95% over 24bp)
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG370.24899057873485866No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.2018842530282638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.1816958277254374No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC270.1816958277254374No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA270.1816958277254374No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA220.1480484522207268No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.13458950201884254No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT190.1278600269179004No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA190.1278600269179004No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA190.1278600269179004No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC190.1278600269179004No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG190.1278600269179004No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG190.1278600269179004No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT190.1278600269179004No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG180.12113055181695827No Hit
CATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAA180.12113055181695827No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC180.12113055181695827No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA170.11440107671601615No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA170.11440107671601615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11440107671601615No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA160.10767160161507401No Hit
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG160.10767160161507401No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC100.0070735877144.639728
ACCGGCC100.0070735877144.63972145
CCTGTAG100.0070735877144.639721
CTAGGGT100.0070735877144.639724
TATCTTG100.0070735877144.639727
GGTATCA202.0872103E-6144.639721
TAAAGGA100.0070735877144.639724
ATGCGGG301.0643271E-7120.533118
AATGCGG301.0643271E-7120.533117
GTATCAA700.092.982691
TGCGGGA405.911061E-790.3998269
AAATGCG451.1914108E-680.355416
TAAATGC502.2288841E-672.319865
CGGTAAA502.2288841E-672.319862
TATCAAC1150.062.8868371
GTAAATG606.5786535E-660.2665564
ATCAACG1353.6379788E-1253.570272
TCAGCAC553.1552062E-452.596264145
TCAACGC1403.6379788E-1251.6570473
CCGGTAA701.6397631E-551.6570471
Produced by FastQC (version 0.11.5)