FastQCFastQC Report
Thu 2 Jun 2016
DRR028488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661289
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26980.16240401278766067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.050.2500081
GTATCAA23350.049.8589171
TCAACGC22750.049.2458234
ATCAACG24150.046.068663
CAACGCA24450.045.218865
AACGCAG26700.041.1220326
GTACATG45300.040.501751
TACATGG45350.038.8299062
TATCAAC31900.036.262252
ACGCAGA29550.036.1584747
CGCAGAG28950.034.3301628
ACATGGG48600.033.80763
CATGGGG35500.027.1988264
GCAGAGT36200.024.850229
GAGTACA30800.022.009481
GTATACG2454.516307E-521.0525441
CATGGGA23550.019.7177284
ACCGTAC1850.005030387519.6065948
GTATTAG13700.018.2865181
GTATTAT15050.018.114981
Produced by FastQC (version 0.11.5)