FastQCFastQC Report
Thu 2 Jun 2016
DRR028488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661289
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.13176515344410275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.1211709702526171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.079.375181
GTATCAA42500.060.3490221
ATCAACG50550.049.5590743
TCAACGC50400.049.275594
CAACGCA51450.048.4106945
AACGCAG54500.045.3029146
TATCAAC58200.044.0401272
ACGCAGA61200.039.27857
CGCAGAG61600.038.670838
GTACATG46750.034.870391
GCAGAGT72400.032.002199
TACATGG50250.031.41152
ACATGGG49750.029.8352073
TACCGTT1450.001236202124.9672247
GTGGTAT12050.024.6520331
CAGAGTA70050.024.0833029
CATGGGG34800.023.0946834
GAGTACA34750.022.5177441
CATGGGA22800.021.5944584
GTCCGTC1750.003687657620.6871289
Produced by FastQC (version 0.11.5)