FastQCFastQC Report
Thu 2 Jun 2016
DRR028487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670314
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27470.16446009552694882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21700.055.359734
ATCAACG22000.053.9165463
GGTATCA14600.052.990191
GTATCAA24700.050.7120251
CAACGCA22700.050.6514175
AACGCAG24050.046.573736
ACGCAGA26650.041.4782077
CGCAGAG26700.039.9591678
GTACATG37650.039.335991
TATCAAC33300.037.169262
TACATGG41850.033.272482
ACATGGG43500.029.639283
CAACGCG1000.00560563329.481725
GCAGAGT33050.028.5484319
GAGTACA29500.025.9758821
CATGGGA21800.025.0173514
AGTACAT32500.021.7623942
AGTACCG2454.5067023E-521.0583725
GTATTAG14150.020.3081
CATGGGG30150.020.2888224
Produced by FastQC (version 0.11.5)