FastQCFastQC Report
Thu 2 Jun 2016
DRR028487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670314
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28600.17122529057410762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.15721594861804428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.078.823141
GTATCAA45400.058.883741
ATCAACG51900.051.0536083
TCAACGC51800.050.872654
CAACGCA53500.049.120825
AACGCAG55400.047.0441326
TATCAAC59200.045.1250152
CGCAGAG64000.040.4963388
ACGCAGA64350.040.2760777
GTACATG39200.032.897191
GCAGAGT75700.032.8028079
TACATGG40500.030.0308062
ACATGGG40350.029.7836063
CAGAGTA74100.023.8387999
CATGGGG25550.023.2346254
GAGTACA30050.019.287251
CATGGGA24200.017.9493454
AGAGTAC68500.017.3326915
TATACAG15050.017.3172445
GTATTAG14700.017.2494621
Produced by FastQC (version 0.11.5)