FastQCFastQC Report
Thu 2 Jun 2016
DRR028486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759140
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28190.16024875791579976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC32200.056.7709664
GTATCAA33400.056.700911
ATCAACG32600.055.6059043
CAACGCA34100.053.6077775
GGTATCA19600.053.011051
AACGCAG36300.049.1318326
GTACATG74150.044.3227921
TACATGG75800.042.286142
TATCAAC44500.041.7271272
CGCAGAG41050.041.3795748
ACGCAGA41900.040.278727
ACATGGG80350.037.1426163
CATGGGA41300.033.3750574
GCAGAGT49450.031.708149
CATGGGG43950.028.0083854
GAGTACA50900.026.7828831
ATGGGAT23900.025.5982745
AGTACAT56800.023.3508192
TATACTG14850.019.854735
GTACGGA3602.2393815E-618.4244376
Produced by FastQC (version 0.11.5)