FastQCFastQC Report
Thu 2 Jun 2016
DRR028486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759140
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.15712223018065644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25180.14313812431074274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.078.139271
GTATCAA52100.061.881061
TCAACGC62150.051.1402054
ATCAACG62400.050.9353183
CAACGCA63750.049.743125
AACGCAG69600.045.666156
TATCAAC71300.044.678882
ACGCAGA77250.040.6752627
CGCAGAG77300.040.5552948
GTACATG77100.040.030471
TACATGG77950.039.195432
ACATGGG79000.037.208153
GCAGAGT89850.034.0848549
CATGGGA39900.032.843154
CATGGGG42100.029.2351934
GAGTACA54500.027.9163271
AGTACAT61050.024.5484332
CAGAGTA87050.024.5353579
GTGGTAT12850.021.988571
ATGGGAT24100.020.4282425
Produced by FastQC (version 0.11.5)