FastQCFastQC Report
Thu 2 Jun 2016
DRR028484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278702
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20660.16157009217159277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.058.9456441
GTATCAA22900.050.1938931
TCAACGC23450.046.516054
ATCAACG24000.045.7442743
CAACGCA24150.045.1677635
AACGCAG24550.044.1245166
GTACATG44250.043.293411
TACATGG44850.039.757092
ACGCAGA28500.037.2348187
TATCAAC30300.036.9626162
ACATGGG47100.036.4499363
CGCAGAG29450.034.5000658
CATGGGG29700.028.0418074
GCAGAGT33450.027.7731699
CATGGGA21800.027.723074
CAGAGTA32700.022.1954449
GTATATA12500.021.8098891
GAGTACA31550.021.4857351
GTGGTAT8850.019.9815751
GTACTAG3751.5257501E-719.6485481
Produced by FastQC (version 0.11.5)