FastQCFastQC Report
Thu 2 Jun 2016
DRR028484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278702
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.22155279337953646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.20004660976521504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.11316162796335659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.086.80561
GTATCAA42500.062.2189981
TCAACGC49750.051.962544
ATCAACG50050.051.5063933
CAACGCA50750.050.938655
AACGCAG53950.048.0514836
TATCAAC55400.047.055242
ACGCAGA59950.042.155237
CGCAGAG60850.041.293738
GTACATG43450.039.5164221
TACATGG45050.037.7735942
GCAGAGT66700.035.9349829
ACATGGG46400.035.113963
CATGGGG30050.029.6398414
CATGGGA22000.026.661074
ACTATCG1801.5330766E-424.1375928
GTGGTAT10700.022.34341
CAGAGTA65550.021.7624999
GAGTACA32350.020.3791541
AGTACAT34450.018.917712
Produced by FastQC (version 0.11.5)