FastQCFastQC Report
Thu 2 Jun 2016
DRR028483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197531
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19140.15982884785446055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13850.053.2390564
ATCAACG13950.052.8460463
CAACGCA15100.048.3476945
AACGCAG15650.046.638546
GTACATG26750.043.5487821
GTATCAA18250.042.4198571
ACGCAGA17300.041.7641877
GGTATCA10800.041.6436541
CGCAGAG17950.038.4489568
TATCAAC19650.038.266992
TACATGG29750.036.178672
ACATGGG33400.030.9006943
GCAGAGT23550.025.604289
CATGGGA16300.022.6184334
GAGTACA21350.021.7563191
TACAGCG2053.274017E-421.578517
CATGGGG24350.021.1972354
ACAGCGT2404.295553E-521.1890858
CCGGTCG1900.00581940319.1179719
GTCTTAC6650.018.8482071
Produced by FastQC (version 0.11.5)