FastQCFastQC Report
Thu 2 Jun 2016
DRR028483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197531
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.14780410695004972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.1417082313526748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.071.214531
GTATCAA34400.059.596481
ATCAACG35350.056.3248633
TCAACGC35600.055.929324
CAACGCA36750.054.3761675
AACGCAG38650.050.9537626
TATCAAC41400.048.7933582
ACGCAGA45050.043.072187
CGCAGAG45400.042.580658
GCAGAGT53750.034.8881659
GTACATG28300.031.2295721
GTGGTAT9700.028.3794861
TACATGG30300.027.9576112
TGTACCG1050.007759553427.5821115
ACATGGG32500.025.1739853
TGGTATC9850.024.9919152
GATCGTG2252.6796652E-522.5253947
CTTAGAC4853.6743586E-1019.4070033
CATGGGG24700.018.7603054
GTACACG1950.00689435918.5648846
Produced by FastQC (version 0.11.5)