FastQCFastQC Report
Thu 2 Jun 2016
DRR028482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514800
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24420.16120940058093478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.055.9884951
ATCAACG20150.049.38533
TCAACGC20350.049.273884
CAACGCA21450.045.7173845
GTATCAA24700.043.5692521
AACGCAG22850.041.9383056
GTACATG37600.041.755721
TACATGG40150.037.4527362
TATCAAC29500.035.481772
ACGCAGA25700.034.7061127
CGCAGAG25400.034.0108768
ACATGGG46200.029.8358613
GCAGAGT30000.024.4410139
GAGTACA28450.023.0585441
CATGGGA21750.023.0511134
CATGGGG32750.022.2877464
CCTATAC6900.020.297543
GTATTAG11600.019.0628341
GTATTAA15350.018.7275281
AGTACAT31200.018.6642882
Produced by FastQC (version 0.11.5)