FastQCFastQC Report
Thu 2 Jun 2016
DRR028482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514800
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.1273435437021389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.11737523105360442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.078.968191
GTATCAA40750.061.177811
ATCAACG46550.052.5707553
TCAACGC46700.052.246864
CAACGCA48250.050.418415
AACGCAG51950.046.1306576
TATCAAC54300.045.6009062
ACGCAGA58750.040.4215747
CGCAGAG58550.039.81778
GCAGAGT70400.031.6756299
GTACATG42600.031.6421851
TACATGG45500.027.8467062
ACATGGG48150.025.7126623
CAGAGTA68800.022.0992779
TATACTG11250.021.2377555
CATGGGA20650.020.6861274
GAGTACA34050.020.4322991
TATATAC17050.018.6842423
TCTATAC11150.018.1815283
GTACGGA2050.00920030617.658896
Produced by FastQC (version 0.11.5)