FastQCFastQC Report
Thu 2 Jun 2016
DRR028481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289282
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20770.1610974170119493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.052.8977281
TCAACGC16100.050.355754
ATCAACG16800.047.370733
CAACGCA17050.047.119615
GTATCAA18400.045.6489331
AACGCAG18600.042.783056
GTACATG31750.040.146341
TATCAAC23250.034.863042
ACGCAGA21650.034.033227
TACATGG34700.033.7647252
CGCAGAG21800.033.6165738
ACATGGG37700.029.5142123
CATGGGG26250.023.58484
GTCTCGC3452.4610927E-923.4918481
GAGTACA23950.022.149841
GCAGAGT30450.021.6833119
GGTACCG2154.5346346E-420.5623956
CAGAGTA25600.019.5772274
ACCGCGG2256.8336795E-419.3481859
GTACCGC2358.254311E-418.8124057
Produced by FastQC (version 0.11.5)