FastQCFastQC Report
Thu 2 Jun 2016
DRR028481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289282
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.17978999163875709No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.16412235647437876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.077.475581
GTATCAA35550.055.045941
TCAACGC37500.051.55374
ATCAACG37750.051.2122883
CAACGCA38000.050.8753625
AACGCAG40950.047.387176
TATCAAC43900.043.708032
ACGCAGA47250.040.6091547
CGCAGAG48600.038.8851788
GCAGAGT56800.031.359329
GTACATG33600.029.1202871
GTGGTAT9300.026.4970251
TACATGG35500.025.6993452
ACATGGG36050.024.7047063
ATGGGCG2755.2709347E-621.063825
TGGTATC10900.020.5927832
CGTAGGA2256.9288205E-419.3085022
GAGTACA26650.019.0371511
TCTATAC8400.018.9637073
GTATAGG5407.4578566E-1118.7903731
Produced by FastQC (version 0.11.5)