FastQCFastQC Report
Thu 2 Jun 2016
DRR028478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6462
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA861.3308573197152584No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA410.634478489631693No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA340.5261528938409161No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC300.4642525533890436No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA230.35592695759826676No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA220.3404518724852987No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT160.24760136180748993No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA130.20117610646858555No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA130.20117610646858555No Hit
GTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATA130.20117610646858555No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA120.18570102135561745No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG120.18570102135561745No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT120.18570102135561745No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC120.18570102135561745No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT110.17022593624264934No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT110.17022593624264934No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC110.17022593624264934No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG100.1547508511296812No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG100.1547508511296812No Hit
GCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCG100.1547508511296812No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC100.1547508511296812No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC90.1392757660167131No Hit
CGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAA90.1392757660167131No Hit
GTATCAACGCAGAGTACATGGGAGCTATACGAAGCATTAGGCGAGCGTGC90.1392757660167131No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG90.1392757660167131No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC90.1392757660167131No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG80.12380068090374496No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80.12380068090374496No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC80.12380068090374496No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC70.10832559579077684No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC70.10832559579077684No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA70.10832559579077684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAG100.0067277667146.41272
CCATTAA100.0067277667146.41271
GGGATAT100.006889975145.259849
TTGAAGT100.006889975145.259849
GCGGGCT100.0070547615144.1258
GTTGAAG100.0070547615144.1258
CAACACA258.618183E-487.847616
AGTACAT501.9361487E-673.2063452
GAGTACA501.9361487E-673.2063451
CATGGGG350.003275950462.7483024
AACGCAG400.00555917854.9047626
ACGCAGA400.00555917854.9047627
GTACATG752.1448137E-548.8042343
TACATGG752.1448137E-548.8042344
CATGGGA450.00885781148.8042346
ATCAACG450.00885781148.8042343
CAACGCA450.00885781148.8042345
ACATGGG803.1415435E-545.7539675
Produced by FastQC (version 0.11.5)