FastQCFastQC Report
Thu 2 Jun 2016
DRR028477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156534
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17570.15191944205704286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.049.0112421
TCAACGC14200.048.9020354
ATCAACG14700.047.221833
CAACGCA15500.044.325955
GTATCAA17100.043.6152271
AACGCAG15850.042.8695566
GTACATG29350.040.2554551
TACATGG30700.038.7275282
ACATGGG32000.034.6157343
ACGCAGA18550.034.2408837
TATCAAC20800.033.728152
CGCAGAG18350.033.2537358
CATGGGA15750.031.4254634
GACGACC10650.028.996595145
GTATAAT11600.022.9169791
GTCTCAC7000.022.153081
GCAGAGT22650.022.1298339
CATGGGG20550.021.9283244
GAGTACA21800.021.3401241
TATACTG9750.019.7004225
Produced by FastQC (version 0.11.5)