FastQCFastQC Report
Thu 2 Jun 2016
DRR028477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156534
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.10185606302970773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.079.662361
GTATCAA27900.059.9796521
TCAACGC29950.055.5986564
ATCAACG30650.054.5650753
CAACGCA30700.054.4762085
AACGCAG33200.049.9379436
TATCAAC34400.047.985462
ACGCAGA37400.043.9427767
CGCAGAG37550.042.4175878
GCAGAGT43750.034.9170579
GTACATG30100.033.213051
TACATGG30950.031.1117342
ACATGGG32650.027.4961413
AGTCGTG1801.5349172E-424.1330387
CATGGGG22550.023.7584674
CCGGTCG2104.3554275E-420.685469
TATACTG9300.020.2406125
CAGAGTA41600.020.1882139
GTATATA10100.018.6486841
GAGTACA25050.018.508381
Produced by FastQC (version 0.11.5)