FastQCFastQC Report
Thu 2 Jun 2016
DRR028476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408268
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20990.14904833454995783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.051.0061721
TCAACGC22000.050.6567424
ATCAACG22900.048.97923
GTATCAA24400.047.7792171
CAACGCA23300.046.880135
AACGCAG25200.042.7488176
GTACATG38200.042.301181
TACATGG39400.038.955672
TATCAAC29850.038.564152
CGCAGAG27650.036.5264478
ACATGGG41850.035.969823
ACGCAGA29450.035.3281977
CATGGGG28000.030.3125744
GCAGAGT33450.026.9328339
GACGACC13000.023.771255145
GTATTAG10950.023.5844481
CATGGGA20500.023.4014364
GAGTACA29150.023.0342771
GTGGTAT10450.021.1824761
TATACTG10700.020.0030965
Produced by FastQC (version 0.11.5)