FastQCFastQC Report
Thu 2 Jun 2016
DRR028476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408268
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.12043162239005643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.11425382100566087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.071.6827551
GTATCAA35700.062.9221081
ATCAACG40000.056.1161083
TCAACGC40000.056.1161084
CAACGCA41300.054.1744235
AACGCAG44200.050.619996
TATCAAC48150.047.0688822
ACGCAGA51050.043.4021767
CGCAGAG50850.043.2880948
GTACATG43700.040.4592821
TACATGG44550.037.5448272
ACATGGG44050.036.4916043
GCAGAGT59950.035.5094539
CATGGGA20550.030.3020824
CATGGGG29200.025.2931614
GTGGTAT10850.023.3748111
TATACTG10600.022.5420765
GAGTACA31850.021.8409521
TGGTATC11000.019.0893522
AGTACAT35250.018.6925322
Produced by FastQC (version 0.11.5)