FastQCFastQC Report
Thu 2 Jun 2016
DRR028475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321477
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20960.15861040335927148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.064.0456851
GTATCAA23650.052.7062261
ATCAACG22800.050.789183
TCAACGC22700.050.6919484
CAACGCA23250.048.8601655
AACGCAG25650.043.7030566
TATCAAC30000.039.580122
ACGCAGA28100.037.0071267
GTACATG36350.035.7120361
CGCAGAG27850.034.9060758
GACGACC10550.033.391582145
TACATGG37750.032.6266172
GCAGAGT31450.027.911699
CATGGGA19100.027.0336634
ACATGGG42300.026.8526253
GAGTACA29050.020.0579871
GATACCG1900.005334154219.4083637
ATACCGT2709.468787E-519.1200896
GTATTAA16300.019.0050091
GTATTAG10500.018.9662231
Produced by FastQC (version 0.11.5)