FastQCFastQC Report
Thu 2 Jun 2016
DRR028475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321477
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30810.23314821218984516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.20272770543868718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15510.11736867156976626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.082.461151
GTATCAA41300.066.3246841
ATCAACG48200.056.4844673
TCAACGC48300.056.06774
CAACGCA49900.054.4150435
AACGCAG52500.051.9960486
TATCAAC55400.049.404932
ACGCAGA59650.045.2779357
CGCAGAG59100.044.96428
GCAGAGT67000.038.257519
GTACATG35050.035.3542441
TACATGG37100.032.2031442
ACATGGG39000.028.9633143
TAGATCG1255.1930576E-428.9633125
GTGGTAT9050.028.025471
ACTATCG1050.00775710227.5841068
TGGTATC9150.026.1144622
CAGAGTA65750.024.448129
CATGGGG24700.024.3315284
CGTACAC1550.001822164723.357513
Produced by FastQC (version 0.11.5)