FastQCFastQC Report
Thu 2 Jun 2016
DRR028474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472020
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21810.1481637477751661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17450.055.8338284
CAACGCA18500.052.2659075
ATCAACG18550.052.119563
GGTATCA13250.050.6908151
GTATCAA21250.049.3211481
AACGCAG21750.044.1058966
CGCAGAG21350.042.1562278
ACGCAGA22850.040.044977
GTACATG34050.038.367181
TATCAAC27500.036.2305072
TACATGG33250.034.6263122
GACGACC11750.033.046986145
ACATGGG37650.029.5995643
GCAGAGT27700.029.086819
CATGGGG26250.023.6194024
GAGTACA24100.022.6630541
ATACCGA1650.002347673522.361386
TATACCG1800.003883465220.5029545
GTATTAG12150.020.046631
CATGGGA19750.019.0599614
Produced by FastQC (version 0.11.5)