FastQCFastQC Report
Thu 2 Jun 2016
DRR028474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472020
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.10414260675806035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.10251219412779718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.072.898971
GTATCAA33100.056.7101481
TCAACGC34250.054.972774
ATCAACG34550.054.7050323
CAACGCA35000.053.5878755
AACGCAG37500.050.015356
TATCAAC42200.044.959732
CGCAGAG42300.042.4566968
ACGCAGA43650.042.139017
GTACATG33750.038.6534041
TACATGG34150.037.109252
GCAGAGT52200.032.6010939
ACATGGG36050.032.341153
CATGGGG22750.026.1016084
CATGGGA18850.021.8976924
CAGAGTA50900.020.3447849
GAGTACA25600.018.6849961
AGAGTAC46150.018.5159135
GTATATA18450.017.6768631
GTACTAA8350.017.3593121
Produced by FastQC (version 0.11.5)