FastQCFastQC Report
Thu 2 Jun 2016
DRR028472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106282
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16970.1533966927058381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.060.03181
GTATCAA21250.055.1467361
TCAACGC20650.054.9824034
ATCAACG21200.052.845693
CAACGCA21250.052.393995
AACGCAG22000.048.922976
TATCAAC25400.044.3976062
GTACATG39400.039.844191
ACGCAGA25800.039.7189677
TACATGG38950.038.6033132
CGCAGAG26650.037.0517548
ACATGGG41900.035.005873
GCAGAGT29100.030.6887479
CATGGGG28400.027.5176014
GAGTACA26600.025.7681331
CATGGGA17850.025.608054
GTCCTAC3051.7089405E-824.1647241
GACGACC10150.024.095732145
GTATATA9600.023.0320011
TATTAGG6600.022.33512
Produced by FastQC (version 0.11.5)