FastQCFastQC Report
Thu 2 Jun 2016
DRR028472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106282
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.21721405572900943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22220.200852947078593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.11136401026139808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.079.5804441
GTATCAA37150.064.172281
TCAACGC44700.053.1351784
ATCAACG45300.052.43143
CAACGCA46350.051.3998645
AACGCAG46950.050.5887646
TATCAAC47500.049.5456542
ACGCAGA55450.042.311587
CGCAGAG57050.040.8710758
GTACATG40400.039.2801481
TACATGG41500.037.1661262
GCAGAGT62250.035.595739
ACATGGG42950.035.2370033
CATGGGG26850.029.3966644
GAGTACA29200.025.312071
CATGGGA20200.023.6596534
CAGAGTA58450.023.5388349
TATACAG10050.020.895265
AGTACAT31750.020.5264852
GTGGTAT8900.019.5403161
Produced by FastQC (version 0.11.5)