FastQCFastQC Report
Thu 2 Jun 2016
DRR028471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12340
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.6320907617504052No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA300.24311183144246357No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA210.17017828200972446No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150.12155591572123178No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA150.12155591572123178No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA140.11345218800648299No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATA100.0066201244147.76252
CGGGTTA100.0066201244147.76256
CAGAGTG100.0070428825144.746939
ACATACA100.0070428825144.746939
AAGTTTC100.0070428825144.746939
GGTATCA300.001752999373.881251
AACACAG300.001752999373.881255
TCGCATT350.003230426263.3267864
CGCATTG350.003230426263.3267865
GCATCGC350.003230426263.3267861
AGTACAT400.005481747455.410947
CAACACA450.00873415449.2541664
GAGTACA450.00873415449.2541666
GCATTGC450.00873415449.2541666
CACAGAA450.00873415449.2541662
CTGCCGA2950.00405396714.660191145
CCCCCCC2853.7565563E-46.602492360-64
Produced by FastQC (version 0.11.5)