FastQCFastQC Report
Thu 2 Jun 2016
DRR028471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12340
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.7536466774716369No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.713128038897893No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT720.5834683954619124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.48622366288492713No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC350.28363047001620745No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.25121555915721233No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA270.2188006482982172No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG240.1944894651539708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.17828200972447325No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT220.17828200972447325RNA PCR Primer, Index 17 (95% over 21bp)
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA210.17017828200972446No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA210.17017828200972446No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG180.1458670988654781No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA180.1458670988654781No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC170.1377633711507293No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA150.12155591572123178No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT140.11345218800648299RNA PCR Primer, Index 17 (95% over 23bp)
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA130.10534846029173421No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA130.10534846029173421No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTGG100.007092991144.40651
TTGAGAG100.007092991144.40657
GCTAAGA100.007092991144.40652
TGGACCA100.007092991144.40655
TGTGGAC100.007092991144.40653
GTGGACC100.007092991144.40654
GGTATCA450.0128.361341
GTATCAA902.5465852E-1064.180671
TATCAAC1006.475602E-1057.76262
ATCAACG1059.986252E-1055.0123
TCAACGC1101.5079422E-952.5114564
AACGCAG1152.237357E-950.2283486
CGCAGAG1152.237357E-950.2283488
CAACGCA1152.237357E-950.2283485
ACGCAGA1203.261448E-948.1354987
GCAGAGT1359.245923E-942.7871139
AGTACTT1500.024.067755
AGAGTAC1400.020.6295013
ACTTTTT1450.019.9181398
GTACTTT1502.2889013E-419.25426
Produced by FastQC (version 0.11.5)