FastQCFastQC Report
Thu 2 Jun 2016
DRR028470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066376
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16220.1521039483259188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16450.056.51861
ATCAACG16050.053.329773
TCAACGC16100.053.194974
CAACGCA16250.053.1582835
GGTATCA10850.050.3255731
AACGCAG17300.049.4908036
TATCAAC20800.042.57012
CGCAGAG18650.042.0776868
ACGCAGA18900.041.786367
GTACATG26150.033.5785561
GCAGAGT23350.029.2514829
TACATGG28400.029.0995622
ACATGGG27900.027.2407933
CGCGATG1600.002290442422.45593145
TATACCG2002.7439743E-422.1492845
GACGACC9050.021.43859145
CATGGGA13500.021.3289414
GAGTACA20650.019.2956961
ATAATAC10800.019.1302663
CCTATAC5405.820766E-1119.1302663
Produced by FastQC (version 0.11.5)