FastQCFastQC Report
Thu 2 Jun 2016
DRR028470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066376
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.1484467017262204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.13775628858864042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.083.2553561
GTATCAA29550.059.098421
TCAACGC33050.051.9238974
ATCAACG33300.051.751523
CAACGCA34300.050.0316245
AACGCAG35750.047.7998286
TATCAAC38100.045.421692
ACGCAGA41050.040.5700047
CGCAGAG40900.040.010648
GCAGAGT47450.032.6563729
GTACATG29150.031.3219131
CTAGCCG1652.41021E-630.7188159
TACATGG29150.029.5596142
TAGCCCG1050.00775625527.5842445
ACATGGG31250.026.6463783
TACGACC2351.34527E-624.649754
GAGTACA22950.022.4177471
CAGAGTA45100.022.1560789
AAGACCG2003.1297887E-421.722595
CATGGGG19250.021.440484
Produced by FastQC (version 0.11.5)