FastQCFastQC Report
Thu 2 Jun 2016
DRR028469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148856
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17330.15084571086367654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17350.052.750764
GTATCAA19000.051.640491
GGTATCA13000.051.0730131
ATCAACG18300.050.800763
CAACGCA18800.048.6822135
AACGCAG19550.045.671766
GTACATG33250.041.489891
ACGCAGA21950.039.335077
TACATGG34000.039.0575872
CGCAGAG22700.038.7161188
TATCAAC25000.037.7730262
ACATGGG38800.032.7075043
GCAGAGT25300.031.2937379
GACGACC9000.027.967138145
CATGGGG26150.025.4025484
GAGTACA25300.023.6187461
CATGGGA17550.022.7103144
TGTACCG1700.00278812121.7083875
GTAGCGT1800.00426205920.1757558
AGTACAT26300.019.636052
Produced by FastQC (version 0.11.5)