FastQCFastQC Report
Thu 2 Jun 2016
DRR028469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148856
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.19445430933032512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.1843573084877478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.082.9265751
GTATCAA35100.060.697731
TCAACGC40900.051.6930924
ATCAACG41700.051.3959123
CAACGCA42650.049.5720375
AACGCAG45300.046.6721276
TATCAAC47450.044.404782
CGCAGAG50950.040.3596238
ACGCAGA51350.040.1862377
GTACATG34400.037.7074171
GCAGAGT58000.034.455149
TACATGG35450.034.109332
ACATGGG35800.032.36013
GAGTACA27500.026.6146581
CATGGGG24900.025.8799614
AGTACAT27900.024.6542822
GTACGGA1851.8471137E-423.4829396
CAGAGTA56950.023.0121829
GGCTACG2003.130841E-421.721727
GCTACGT1700.003116521.2958038
Produced by FastQC (version 0.11.5)