FastQCFastQC Report
Thu 2 Jun 2016
DRR028468_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028468_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458669
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38390.15614139194824517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24050.050.2125744
ATCAACG25950.045.6686443
CAACGCA26150.045.337345
GTATCAA31850.041.137711
GGTATCA21150.039.3276181
AACGCAG31300.037.8713576
GTACATG48550.035.3261451
CGCAGAG31150.032.8180548
TACATGG51600.031.2402782
ACGCAGA36150.031.1623767
TATCAAC40350.031.0117232
ACATGGG50250.030.322423
TACGTCG1305.9210096E-428.32235
GAGTACA35700.022.4743861
CATGGGA30150.021.9805684
GCAGAGT41600.021.6117049
GTATATA26400.019.5174771
ATACGCC3952.6598173E-718.6394126
GTATAAT24250.017.6053961
TATATAC28500.017.5627943
Produced by FastQC (version 0.11.5)