FastQCFastQC Report
Thu 2 Jun 2016
DRR028468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458669
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC36350.055.1866574
CAACGCA37750.053.1400035
ATCAACG37700.053.0183833
GTATCAA41150.050.5534441
GGTATCA24150.050.423251
AACGCAG42800.047.0391856
CGCAGAG46650.041.759898
ACGCAGA50700.039.138247
TATCAAC52450.038.936992
GCAGAGT61300.029.298819
GTACATG53300.029.1021611
TACATGG55350.026.9530752
ACATGGG51850.026.537723
CCGTGCG1658.486938E-526.3345589
CCGTGTA3757.876224E-921.2432129
ATAATAC26650.019.2938943
GAGTACA42100.018.9386621
GTATATA26750.018.696661
CATGGGA29650.018.318734
TCTATAC18900.018.0092163
Produced by FastQC (version 0.11.5)