FastQCFastQC Report
Thu 2 Jun 2016
DRR028467_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028467_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948515
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31340.16084043489529204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18550.053.151964
ATCAACG19750.049.1618353
CAACGCA20150.048.932745
AACGCAG23100.042.673656
GTACATG40900.039.7425651
GTATCAA26250.039.507241
ACGCAGA24250.039.1341867
CGCAGAG24150.036.036798
TACATGG42900.034.977932
TATCAAC29900.033.4571462
GGTATCA17550.032.689211
ACATGGG46400.031.5468713
GCAGAGT33200.023.8106029
CATGGGA26900.023.250114
CATGGGG30250.021.648264
GTATATA20550.021.474661
GAGTACA29500.019.9459571
GTATTAG17050.018.980831
GTATAAT17950.018.0291441
CTATACT15250.016.8871574
Produced by FastQC (version 0.11.5)