FastQCFastQC Report
Thu 2 Jun 2016
DRR028467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948515
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.055.844371
TCAACGC32050.054.9117854
ATCAACG32850.053.7949833
CAACGCA33500.052.5350045
GTATCAA34900.052.344971
AACGCAG36750.048.4802746
CGCAGAG41200.041.486058
TATCAAC43600.041.195762
ACGCAGA42450.040.9468847
GTACATG46700.034.461641
ACATGGG46700.031.7924733
TACATGG49350.031.1125772
GCAGAGT53750.030.047879
GAGTACA35450.023.5169751
CATGGGA25750.023.0634334
TATATCG1600.002189048322.632755
CATGGGG28700.022.4592594
GTATATA20850.019.470711
TATACTG19000.019.0591585
TGGACCG2359.268731E-418.4914385
Produced by FastQC (version 0.11.5)