FastQCFastQC Report
Thu 2 Jun 2016
DRR028466_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028466_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220221
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19450.15939735506928662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16700.047.625424
ATCAACG17350.046.2539563
GGTATCA11300.045.600371
CAACGCA18100.043.943535
GTATCAA19700.043.345151
AACGCAG19100.041.246836
GTACATG30950.040.4330981
TACATGG31250.038.1636852
ACGCAGA21200.035.077267
CGCAGAG20850.034.1086048
TATCAAC25000.033.569912
ACATGGG34250.033.534043
CATGGGG24600.027.840654
GCAGAGT24850.024.8230119
GAGTACA22600.022.8001841
ACACCGT2805.3300737E-621.0364326
GTATATA12250.021.0320071
GTATAAA13350.020.4018361
CAGAGTA24800.019.8984479
GTCTACG3751.5409569E-719.6298751
Produced by FastQC (version 0.11.5)