FastQCFastQC Report
Thu 2 Jun 2016
DRR028466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220221
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.074.201451
GTATCAA29050.059.881481
TCAACGC30400.055.736844
CAACGCA31500.053.790485
ATCAACG31950.053.0328643
AACGCAG33100.050.9715776
TATCAAC37700.045.9046482
ACGCAGA37850.043.8096437
CGCAGAG37800.043.101358
GCAGAGT43700.035.7908829
GTACATG30500.035.646661
TACATGG31150.033.4737632
TGTACGA1352.1608075E-532.182342
GTACGAC1352.1608075E-532.182343
ACATGGG32950.031.4253923
GGTGCGT1801.5333822E-424.1367536
CATGGGG23550.022.7531174
TATACGG1600.002191137322.6282082
CATGGGA16000.021.2705144
CAGAGTA43350.021.2136179
Produced by FastQC (version 0.11.5)