FastQCFastQC Report
Thu 2 Jun 2016
DRR028465_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028465_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425688
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22440.15739769150052466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.052.7212031
TCAACGC17200.050.9622574
ATCAACG18200.048.148253
GTATCAA19800.046.854111
CAACGCA18900.045.5988855
AACGCAG20500.042.032376
ACGCAGA21300.038.0333867
TATCAAC24450.035.5392342
CGCAGAG22800.034.355128
GTACATG31050.033.6720121
TACATGG32550.032.3511662
ACATGGG34850.028.1030713
GAGCGCG2001.0633294E-525.3828149
GCAGAGT26900.023.9943779
GAGTACA23800.023.5114251
GTATACG1650.002378304322.311481
GTATAAT13000.022.0883661
CATGGGG25600.019.2781354
GTATTGG10600.018.7542721
CTATACT10050.018.3233174
Produced by FastQC (version 0.11.5)