FastQCFastQC Report
Thu 2 Jun 2016
DRR028465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425688
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.065.006541
GTATCAA31100.055.4641341
TCAACGC31200.053.849114
ATCAACG32400.052.3017233
CAACGCA33450.050.226985
AACGCAG34150.049.4094926
TATCAAC37550.045.5142332
ACGCAGA39300.042.0133637
CGCAGAG39350.041.407888
GTACATG34550.033.77331
GCAGAGT48300.032.5355229
TACATGG36050.030.7348652
ACATGGG35150.028.4314443
TCGTGCG1100.0097265926.3337359
GACCCGT1450.001234840824.9716476
GTATAGG5350.024.3845271
CAGAGTA45150.022.294739
CATGGGA18600.021.4138574
GAGTACA25850.020.1868061
GTATTAA15400.019.2956221
Produced by FastQC (version 0.11.5)